Evaluation Role of IL-13 and Eosinophils in Adult Asthmatic Patients

Abstract

 Background Asthma is a chronic inflammatory disease primarily re- sulting from interactions between genetic and environmental factors, in which immunological mediators and cells play a crucial role. This study aimed to determine the role of IL-13 and eosinophilia in adult asthmatic patients.Methods This case-control study was conducted on 69 patients (48 fe- males, 21 males) with acute bronchial Asthma and their age range (18-70). Other 20 healthy subjects (11 females and 9 males) were included as a control group. The optical density of IL-13 in serum was detected by ELISA technique and from which IL-13 was evaluated according to stan- dard carve. A complete blood count was performed for all blood samples to detect eosinophils number (cell/μl) by the RUBY system.Results The results of the study showed that 31.9% of the patients were in the age group of (20-35) years, 27.5% of the patients had clini- cal manifestations in the age >20 years, and 69.6% of patients were fe- males. Significant association of gender and age for cases and controls not demonstrated (p-value = 0.23 and 0.83 respectively). This study detected the distinct role of IL-13 and eosinophil in cases of Asthma compared to healthy controls (P value <0.001) and showed the gradual high of IL-13 in serum associated with an ordered increase in eosinophil count in peripheral blood.Conclusion The results of the study showed that 31.9% of the patients were in the age group of (20-35) years, 27.5% of the patients had clini- cal manifestations in the age >20 years, and 69.6% of patients were fe- males. Significant association of gender and age for cases and controls not demonstrated (p-value = 0.23 and 0.83 respectively). This study detected the distinct role of IL-13 and eosinophil in cases of Asthma compared to healthy controls (P value <0.001) and showed the gradual high of IL-13 in serum associated with an ordered increase in eosinophil count in peripheral blood.